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dc.contributor.authorChidzwondo, F.
dc.contributor.authorDietrich, A.
dc.contributor.authorSithole-Niang, I.
dc.identifier.citationChidzwondo, F., Dietrich, A. and Sithole-Niang, I. (2000) The Mae I assay for scoring atrazine resistance is codon-usage dependent in legumes, Journal of Applied Science in Southern Africa, vol. 6, no.1, pp. 17-25. Harare: UZ Publications.en
dc.descriptionA research experiment on herbicide atrazine usages as observed out in Zimbabwe.en
dc.description.abstractNine different cowpea varieties were grown and total deoxyribonucleic acid (DNA) isolated from the young leaves. This DNA was used to amplify a 291 base pair fragment that contained codon 264 of the psb A gene. The primers used were designed based on the soybean psb A gene sequence. The 291 base pair fragment was digested with the restriction enzyme Mae I to assay for sensitivity/resistance to atrazine using a published method. When Mae I did not cut the 291 base fragment, the whole gene was amplified by the polymerase chain reaction, cloned into pBSK+ll (Stratagene, CA, USA) and sequenced around codon 264. Codons 263 and 264 were found to be GCA and AGT respectively. UWGCG and BLASTsearchesofthepsbAgenesequences in the databases confirmed that Mae I has a recognition site that overlaps with codons 263 and 264 in some legumes but not in others. The Mae I assay used by Cheung and co-workers is therefore not universal but depends on codon usage in legumes.en
dc.publisherUniversity of Zimbabwe (UZ) Publicationsen
dc.subjectScience and Societyen
dc.titleThe Mae I assay for scoring atrazine resistance is codon-usage dependent in legumesen
dc.rights.holderUniversity of Zimbabwe (UZ)en

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